Journal: Communications Biology
Article Title: Persistent intraocular Ebola virus RNA is associated with severe uveitis in a convalescent rhesus monkey
doi: 10.1038/s42003-022-04158-2
Figure Lengend Snippet: a Complete timeline starting with baseline (BL) assessment and ending at necropsy on day 99. Presence of ocular clinical signs and collection of aqueous humor through anterior chamber paracentesis are indicated with dots that are called out with indicator lines. EBOV GP genome equivalents (GEq) in sera, determined by RT-qPCR (red triangles), along with rhesus monkey-specific anti-EBOV GP IgG antibodies in serum detected by ELISA (orange dots). LOD, limit of detection of assay. b Clinical euthanasia scores recorded via cageside observation. Therapeutic human monoclonal antibody 1C3 dosing schedule is indicated with arrows. c Concentrations of aspartate aminotransferase (AST) and platelet counts determined in blood are shown over time. d Edema, eyelid splinting, anterior chamber fibrin clot, and iris vessel dilation visible from day 21 through day 30. e Photographs of the right (OD) and left (OS) eyes, documenting vitreous haze OS with improvement over time. f Precontrast and post-contrast magnetic resonance (MR) images of the globes. Volumetric measurements of both eyes extrapolated from MR scans show decreased eye volume OS, suggestive of hypotony. D; day.
Article Snippet: After intramuscular (IM) inoculation with 1000 plaque-forming units (PFU) of Ebola virus/H. sapiens-tc/COD/1995/Kikwit-9510621 (EBOV; NR-50306, Lot 9510621, BEI Resources, USA), the monkey was administered 25 mg/kg of human monoclonal antibody (mAb) 9.20.1C3 anti-EBOV glycoprotein (GP) 1 and 2 (GP 1,2 ) (“1C3”; Zalgen Labs, Germantown, MD, USA) intravenously (IV) on days 4 and 7 post-exposure.
Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Detection Assay
